APPLICATION NOTE: DNA and RNA absorbance measurements
Absorbance measurements made through microplates are subject to pathlength variability and are vulnerable to interference from surface effects at the air/liquid interface. Modern microplate readers, with smaller optical beams than older wide-beam plate readers, are much more vulnerable to spurious readings due to dust. Particles in the light beam at the time of a read can cause artifactual absorbance spikes of up to 0.3 OD. Therefore it is especially important that sample solutions be free of particles. All of the above factors must be kept in mind in order to get accurate and reproducible absorbance results in microplates.
By following the link below, you will find guidelines for optimizing DNA/RNA absorbance measurements in SpectraMax® microplate readers from Molecular Devices.
Application Note : DNA and RNA absorbance measurements using SpectraMax Microplate Readers.
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